Composite

Part:BBa_K1173501:Design

Designed by: paul james   Group: iGEM13_Exeter   (2013-09-20)


CcaS, CcaR, PcpcG2, cI(lambda), magenta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 667
    Illegal NheI site found at 2478
    Illegal NheI site found at 2501
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2934
    Illegal XhoI site found at 2313
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1331
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To prevent the accidental transcription of CcaR and the magenta pigment, these genes were reverse complement-ed. This should prevent unwanted production of magneta pigment. We also had to deconstruct BBa_C0051, as we needed the cI promoter to go before the magenta pigment.


Source

Synthesised by IDT (gBlocks). Based on a variety of BioBricks.

References